A METHOD FOR TRACKING PARTICLES AND LIFE FORMS IN THREE DIMENSIONS AND IN TIME

摘要

A method for tracking particles and life forms in three dimensions and in tune. The present invention applies a numerical reconstruction approach to digital in- line holographic microscopy images in order to generate a time sequence hologram representing the trajectory of objects such as particles and life forms. By subtracting consecutive (in time) holograms of a particle sample volume and then adding the resulting differences, a final hologram is constructed that contains the tim e evolution of the object trajectory free from spurious background interference effects.