| 摘要 |
<p>A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases ti me for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will secti on them. The apparatus (10) includes a cell flow pathway (16) defined by an inflow tube (20) for delivering cell fragments from a cell sample (14) to a sample port (26). The sample port (26) is in fluid communication with a tiss ue cassette (30) having attached thereto a filter (40). The cell flow pathway (16) is in communication with a reagent flow pathway (18) for delivering the reagents (82, 92, 102) through the sample port (26) to the cassette (30). Th e apparatus (10) is configured such that the application of pressure directs t he cell fragments from the cell sample (14) through the cell flow pathway (16), and effects delivery of the reagents (82, 92, 102) through the reagent flow pathway (18). The apparatus (10) produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick an d efficient manner.</p> |
