发明名称 |
Nucleic acids comprising regions of the rat PEG-3 promoter that display elevated expression in human cancer cells and uses thereof |
摘要 |
This invention provides an isolated nucleic acid comprising a PEG-3 promoter comprising the nucleotide sequence of -270 to +194 of FIG. 2. The invention also provides a method for identifying an agent that modulates PEG-3 promoter activity using a cell which comprises a PEG-3 promoter operatively linked to a reporter gene, wherein reduced reporter gene expression in the presence of the agent is indicative of an agent that inhibits PEG-3 promoter activity and wherein increased reporter gene expression in the presence of the agent is indicative of an agent that enhances PEG-3 promoter activity. The invention provides a method for treating cancer in a subject which comprises administering a nucleic acid comprising a PEG-3 promoter operatively linked to a gene-of-interest, wherein the gene-of-interest is selectively expressed in cancerous cells in the subject and such expression results in growth suppression or death of the cancerous cells.
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申请公布号 |
US6737523(B1) |
申请公布日期 |
2004.05.18 |
申请号 |
US20000621781 |
申请日期 |
2000.07.21 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
发明人 |
FISHER PAUL B.;SU ZAO-ZHONG |
分类号 |
A61K35/12;A61K38/00;A61K39/00;A61K48/00;C07K14/47;C12N15/12;C12N15/85;C40B30/04;G01N33/574;G01N33/68;(IPC1-7):C07H21/04;C12N15/00 |
主分类号 |
A61K35/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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