Multi-dimensional separation of mixtures of bio-substances, e.g. for use in biochemistry, involves forming fraction bonds with selected solutions, using pipette robot in micro-titration plate

摘要

Multi-dimensional separation of a mixture of bio-substances, for analysis by mass spectrometry comprises forming fraction bonds of the substances from solutions at fixed surfaces. Alternatively, fraction solutions of bio-substances are used with surface-bonded mixtures of bio-substances. A given mixture of separation solutions is used to give separation into a number of discrete fractions. Two or more separation generations have different separation dimensions, for the fractions to be divided into a number of sub-fractions. An Independent claim is included for an apparatus for use in the above method, which has a micro-titration plate (2) to take the pipette (4-7) of a robot pipette system. The pipette leaves only a small gap between the pipette point and the inner wall (3) of the well for a fluid flow.