| 摘要 |
PURPOSE: A method for analyzing Mancozeb using HPLC is provided, thereby rapidly and accurately analyzing Mancozeb which is agricultural chemical remained in fruits or leaf vegetables. CONSTITUTION: The method for analyzing Mancozeb using HPLC comprises the steps of: extracting fruits or leaf vegetables containing Mancozeb with 100 ml of 0.03M EDTA disodium salt solution having pH 9.5 to 10.5 for 5 minutes; absorbing the extracted solution with GF/C filter paper, and washing the remained solution with distilled water; regulating the extracted solution to pH 7.2 to 8.0, adding 7 ml of 0.41M tetrabutylammoniumhydrogensulfate and 60 ml of 0.05M chloroform of iodomethane/hexane(5:1) into the extracted solution, stirring them and stationing it for 30 minutes to methylate Mancozeb in the extracted solution; separating the extracted solution into a water-soluble layer and organic solvent layer, adding 0.05M chloroform of iodomethane/hexane(5:1) into the water-soluble layer, separating the organic solvent layer, and mixing the organic solvent layer with the pre-separated organic solvent layer; and adding 4.0 ml of 20 wt.% 1,2-propanediol into the organic solvent layer, concentrating the organic solvent layer, treating methylated Mancozeb containing concentrated solution with acetonitrile and subjecting the acetonitrile treated concentrated solution to HPLC.
|
