SPHEROIDAL HDL PARTICLES WITH A DEFINED PHOSPHOLIPID COMPOSITION

摘要

Spheroidal reconstituted HDL (High Density Lipoprotein) particles with a defined phospholipid composition have been formed for the first time. The spheroidal HDL particles are formed from discoidal HDL particles of defined phospholipid composition to which is added in multiple stepwise format unesterified cholesterol carried in a solvent followed by addition of a LCAT (lecithin: cholesterol acyltransferase). Lysophospholipids are removed and a specific sequence of addition of reagents are found to be beneficial. Additionally it is found that a ratio of phospholipids to apoprotein A-1 of about 100:1 enhances the result.