ARABINOFURANOSIDASE PRODUCED FROM THERMOTOGA MARITIMA AND METHOD FOR PRODUCING ARABINOSE USING THE SAME

摘要

PURPOSE: Arabinofuranosidase produced from Thermotoga maritima and a method for producing arabinose using the same are provided, thereby specifically digesting the side chains of arabinoxylan to inhibit the production of by-products, and improving the production rate and yield of arabinose by conducting the enzyme reaction at high temperature. CONSTITUTION: Alpha-L-arabinofuranosidase produced from Thermotoga maritime has optimal operation temperature of 60 to 120 deg. C and optimal operation pH of 4 to 9, and specifically digests arabinoxylan to produce arabinose, wherein the alpha-L-arabinofuranosidase has the amino acid sequence set forth in SEQ ID NO: 2; and a gene encoding the alpha-L-arabinofuranosidase has the nucleotide sequence set forth in SEQ ID NO: 1. A method for producing arabinose comprises digesting the substrate containing arabinose with the alpha-L-arabinofuranosidase of Thermotoga maritime.