USE OF A CELL MEMBRANE PERMEANT CALCIUM BUFFER FOR REDUCING INJURY OF MAMMALIAN CELLS IN VIVO

摘要

A method of reducing the damaging effect of an injury to mammalian cells by treatment of the cell or mammalian tissue in vivo with a cell membrane permeant calcium buffer. The method comprises treating mammalian tissue with a damage reducing effective amount of the calcium buffer, preferably, a BAPTA derivative. The method may be used to control the concentration of Ca2+ ions in the vicinity of ion channel pores of the cells to prevent diffusion of toxic amounts of Ca2+ ions to subcellular sites located near the source of Ca2+ influx. The buffer treatment may be applied as a prophylactic or after the mammalian tissue has sustained injury. Pharmaceutical compositions comprising the calcium buffer and method of manufacture therefor are described.