| 摘要 |
The invention relates to a method for analyzing gene expression, enabling a reliable correlation of the amount of mRNA transcribed by the gene to be examined, taking into account the translation state present in a cell type, tissue or organism, with the amount of protein which is translated from said mRNA. Determination of the translation efficiency of all mRNA variants which are transcribed by a gene to be examined, coding for a specific protein enables inter alia identification of the preferentially translated mRNA variant in a specific cell type, tissue or organism. It is possible to make a reliable forecast of the amount of protein expressed in a cell type, tissue or organism on the basis of the amount and translation efficiency of the preferentially translated mRNA variant coding for a protein to be examined. |
