发明名称 |
SYSTEM AND METHODS FOR ELECTROPHORETIC SEPARATION OF PROTEINS ON PROTEIN BINDING MEMBRANES |
摘要 |
Proteins can be rapidly separated to a high degree of resolution by electrophoresis on polymeric membranes that have high protein binding capacity. The electrophoretic separation is carried out in a low conductivit y, water-miscible organic solvent buffer. The low conductivity of the organic solvent buffer minimizes heat generation, and the water-miscible nature of t he organic solvent buffer permits the analysis of hydrophobic and low molecular weight proteins as well as hydrophilic proteins. When electrophoresis is conducted under non-denaturing conditions, it allows the detection of enzymatic activities, protein-protein interactions and protein~ligand interactions.
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申请公布号 |
CA2498354(A1) |
申请公布日期 |
2004.03.25 |
申请号 |
CA20032498354 |
申请日期 |
2003.09.09 |
申请人 |
TEMPLE UNIVERSITY - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION |
发明人 |
CHANG, FRANK N.;YONAN, CHRISTOPHER R. |
分类号 |
G01N33/53;C07K1/26;C12M1/34;G01N;G01N21/78;G01N27/447;G01N27/453;G01N33/557;(IPC1-7):G01N27/447 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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