METHOD OF AMINOACYLATING TRNA

摘要

It is intended to provide a method of chemically synthesizing an aminoacyl tRNA which is completely different from the existing methods, namely, a high ly efficient and practically usable method of synthesizing an aminoacyl tRNA whereby any unnatural amino acid can by conveniently aminoacylated without a need for genetic engineering techniques and detected without resort to a radioactive isotope. The above-described aminoacylation method comprises enclosing a tRNA and an amino acid in the vicinity of the interface of a micelle and bringing them close to each other to thereby react the same, or providing between them a peptide nucleic acid specifically binding to the tR NA as an antisense molecule and bringing them close to each other to thereby react the same.