Способ получени интактной козьей зиготы; способ получени трансгенной по hG-CSF козьей зиготы, предусматривающий использование указанной интактной козьей зиготы; способ получени трансгенной по hG-CSF козы, предусматривающий использование указанной трансгенной по hG-CSF козьей зиготы; способ продуцировани hG-CSF, предусматривающий использование указанной трансгенной по hG-CSF козы, и молочна композици , содержаща указанный hG-CSF

摘要

A goat zygote (I) which comprises a nucleic acid construct containing a nucleotide sequence of a goat beta -casein promoter and a nucleic acid sequence encoding human granulocyte-colony stimulating factor (hG-CSF). Independent claims are also included for the following: (1) a method for preparing (I), comprising microinjecting a nucleic acid construct containing a nucleotide sequence of a goat beta-casein promoter and a nucleotide sequence encoding hG-CSF into an intact goat zygote; (2) a method (M1) for preparing an intact goat zygote, comprising synchronizing a female goat, superovulating the female goat, mating the superovulated female goat with a male goat and recovering a zygote from the mated female goat, characterized in that the synchronizing step is conducted by administering norgestomet and estradiol to the female goat, inserting an implant containing norgestomet to the female goat and removing the implant, and the superovulating step is conducted by administering to the female goat, sequentially at predetermined time intervals, a combined dose of pregnant mare serum gonadotropin (PMSG) and follicle stimulating hormone (FSH), divided doses of FSH, and a combined dose of FSH and human chorionic gonadotropin (hCG); (3) a transgenic goat producing milk containing hG-CSF, which is developed from (I); (4) a process for preparing the transgenic goat of (3) into a female goat and allowing the goat zygote to develop to term; (5) a method for producing hG-CSF, comprising producing milk from the transgenic goat of (3) and recovering the hG-CSF from the milk; and (6) a milk composition comprising hG-CSF, which is produced from the transgenic goat of (3); (7) hG-CSF produced by the method of (5). ACTIVITY : None given. No biological data given. MECHANISM OF ACTION : Human granulocyte-colony stimulating factor. No biological data given.