Method for the identification of fungizids using ribose 5-phosphate isomerase

摘要

Identifying fungicides (A) comprises: (a) contacting ribose-5-phosphate isomerase (RPI), or a host cell that contains it, with at least one test compound; (b) comparing RPI activity in presence and absence of the test compound; and (c) identifying compounds that inhibit RPI activity. Independent claims are also included for: (1) (A) identified by the new process; (2) a nucleic acid (I) encoding a polypeptide (II) with RPI activity, derived from a phytopathogenic fungus; (3) a DNA construct containing (I) and a heterologous promoter; (4) a vector containing (I) or the construct of (3); (5) a host cell containing (I), the construct of (3) or the vector of (4); (6) an RPI polypeptide (II) from phytopathogenic fungi, encoded by (I); and (7) identifying compounds that alter expression of (II), by contacting a cell of (5) with at least one test compound and measuring any change in the concentration of (I) caused by the test compound.