Assay for methylation in the GST-Pi gene

摘要

A diagnostic or prognostic assay is disclosed for a disease or condition characterised by abnormal methylation of cytosine at a site or sites within the glutathione-S- transferase (GST) Pi gene and/or its regulatory flanking sequences (e.g. prostate cancer and liver cancer). The assay comprises: (i) isolating DNA from said subject, and (ii) determining (e.g. by selective PCR amplification) the presence of abnormal methylation of cytosine at a site or sites within the GST-Pi gene and/or its regulatory flanking sequences.