| 摘要 |
The invention provides methods for in vitro generation of a telomerase-immortalized spermatogonial cell line. The invention further provides for the generation of spermatocytes and spermatids from immortalized spermatogonial cells by differentiation in the presence of stem cell factor. This differentiation can occur in the absence of supportive cells. Immortalized spermatogonial cell lines provide powerful tools for elucidating molecular mechanisms of spermatogenesis and for screening and developing compounds which affect spermatogenisis. Furthermore, through genomic modification and transplantation techniques, this male germ cell line can be used to generate transgenic animals and in germ cell gene therapy. |
