METHOD FOR PREPARATION OF VESICLES LOADED WITH IMMUNOSTIMULATOR Y OLIGODEOXYNUCLEOTIDES

摘要

The present invention is based on the finding of a simple and fast method for an effective entrapment of active ISS-ODN in liposomes, with more than 60% loading). The method is based on drying a suspension of amphipathic material and then hydrating it with an aqueous solution containing the ISS-ODN thereby entrapping it in liposomes formed from the lipid. Thus, the present invention concerns an efficient method for loading, and retaining, ISS-ODN in liposomal vesicles.Animals treated with liposomal ISS-ODN of the invention are shown to demonstrate a slower tempo of disease development as compared to free ISS-ODN or to liposomes loaded with mutant ISS-ODN (lip M-ODN).