METHOD OF DETERMINING THE CLEAVAGE ACTIVITY OF AN ENDONUCLEASE

摘要

The invention relates to a method of testing the cleavage activity of an endonuclease in relation to a DNA substrate. The inventive method is based on the discrimination of the cleaved DNA substrate and the intact substrate. The aforementioned discrimination can be performed by means of selective degradation or depletion of the intact substrate or by selective amplification of the cleaved substrate. Said method can be applied to the screening and the selection of an endonuclease or a cleavage site for an endonuclease.