发明名称 |
In vitro preparation of homologous heart valves, useful for replacement of diseased valves, by inoculating biodegradable carrier with fibroblasts and attachment to a non-degradable stent |
摘要 |
An in vitro method for preparing homologous heart valves (A), is new. An in vitro method for preparing homologous heart valves (A) comprises: (a) preparation of a biodegradable carrier (scaffold); (b) seeding the carrier with homologous fibroblasts and/or myofibroblasts to form a connective tissue matrix; (c) optionally seeding the matrix with endothelial cells and (d) fixing the matrix to a framework (stent) that is not (easily) degraded. The matrix, before and/or after fixing, is introduced into a pulsatile flow chamber and the flow rate in the chamber increased (dis)continuously. Independent claims are also included for: (1) similar method in which the carrier and stent are fixed together before seeding with cells; (2) autologous heart valves prepared by the method; and (3) autologous heart valves having a connective tissue inner structure, surrounded by a layer of endothelial cells and fixed to a stent.
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申请公布号 |
DE10235237(A1) |
申请公布日期 |
2004.02.12 |
申请号 |
DE20021035237 |
申请日期 |
2002.08.01 |
申请人 |
SYMETIS AG, ZUERICH |
发明人 |
HOERSTRUP, SIMON PHILIPP;ZUEND, GREGOR |
分类号 |
A61F2/24;A61L27/18;A61L27/38;A61L31/00;(IPC1-7):C12N5/08;A61L27/50 |
主分类号 |
A61F2/24 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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