发明名称 |
Immobilization of biomolecules on substrates by attaching them to adsorbed bridging biomolecules |
摘要 |
An assay article for detection first biomolecules contained in a sample is described. The assay article includes a substrate having a modified surface and a first biomolecule directly adsorbed and immobilized on the modified surface of the substrate without linking moieties. A second biomolecule is bound to or adsorbed on the first biomolecule. Also disclosed is a method of making the assay article. A first biomolecule (other than an adhesive protein) is contacted with a modified surface of a substrate. The substrate is dried to directly adsorb the first biomolecule and immobilize it on the modified surface of the substrate without additional fixing steps to form an activated substrate. Then, a second biomolecule is contacted with the activated substrate under conditions sufficient for the first biomolecule to bind the second biomolecule.
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申请公布号 |
US2004029156(A1) |
申请公布日期 |
2004.02.12 |
申请号 |
US20030427658 |
申请日期 |
2003.05.01 |
申请人 |
BECKMAN COULTER, INC. |
发明人 |
MATSON ROBERT S.;RAMPAL JANG B. |
分类号 |
G01N33/53;C07B61/00;C07H21/00;C12M1/00;C12N15/09;C12Q1/68;C40B40/06;C40B40/10;C40B60/14;G01N33/543;G01N33/545;G01N37/00;(IPC1-7):B05D3/00;C12M1/34 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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