METHODS AND APPARATUS FOR SCREENING AND DETECTING MULTIPLE GENETIC MUTATIONS

摘要

An assay system and methods are described where patient samples containing genomic DNA are analyzed for the presence of known genetic polymorphisms usi ng a universal reporter strategy. In a preferred embodiment, the amplified DNA is localized at test sites in an array of sites on a microchip followed by a series of hybridization reactions that screen for the presence of a single mutation from among a number of mutations, and allow the identification of specific mutations. In addition to universal reporters, the assay may use blockers and discriminators for screening and identification of known polymorphisms.