| 摘要 |
The present invention relates to a recombinant host cell line and the use thereof in a recombinant host cell line-based screening system in which the host cell genes of the deoxythymidine triphosphate synthesis pathway are functionally complemented by viral, fungal or mammalian homologues. The present invention also relates to the use of a recombinant host cell line for testing inhibitors of enzymes of the thymidine phosphorylation pathway(s), testing improved phosphorylation of nucleoside analogues, their incorporation into DNA as well as mutants of the involved kinases. In addition, the present invention relates to the use of the recombinant host cell line for the expression, characterisation and purification of any enzyme activity able to complement the said recombinant host cell line for growth. |
