Molecule for assigning genotype to phenotype and components thereof as well as method for constructing assigning molecule and method for utilizing assigning molecule

摘要

An assigning molecule obtained by ligating a genotype molecule to a phenotype molecule which is a protein encoded by the ORF region in the genotype molecule, by transpeptidation, the genotype molecule being constructed by bonding (a) a spacer molecule comprising a donor region which can be bonded to a 3'-terminal end of nucleic acid, a PEG region that is bonded to the donor region and comprises polyethylene glycol as a main component and a peptide acceptor region which is bonded to the PEG region and comprises a group which can be bonded to a peptide by transpeptidation, to (b) a 3'-terminal end of a coding molecule which is a nucleic acid comprising a 5' untranslated region comrising a transcription promoter and a translation enhancer; an ORF region which is bonded to the 3'-terminal side of the 5' untranslated region and encodes a protein; and a 3'-terminal region that is bonded to the 3'-terminal side of the ORF region and comprises a polyA sequence.