发明名称 |
DETECTION OF CHEMICAL LIGATION USING FLUORESCENCE QUENCHING LEAVING GROUPS |
摘要 |
<p>Novel compounds having a fluorescence quencher as a leaving group are disclosed. Nucleic acids and other molecules containing a fluorophore and a fluorescence quencher are disclosed as an embodiment of this invention. The use of the oligonucleotides in enzyme-free oligonucleotide ligation reactions results in an increase in fluorescence when the oligonucleotide also contains a nearby fluorophore. The ligation reactions can be performed in solution, on surfaces, or in cells.</p> |
申请公布号 |
WO2004010101(A2) |
申请公布日期 |
2004.01.29 |
申请号 |
WO2003US22320 |
申请日期 |
2003.07.17 |
申请人 |
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY |
发明人 |
KOOL, ERIC T., |
分类号 |
C07H21/04;C07K14/00;C12Q1/68;G01N33/53;G01N33/533;G01N33/542;G01N33/58;(IPC1-7):G01N/ |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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