发明名称 |
Methods and kits for transferases |
摘要 |
A method for detecting transferase activity of a sample includes contacting the sample with a substrate and at least one of a phosphate group donor and a phosphate group acceptor. The substrate includes a reporter compound and amino acids. A peptidase is added that cleaves a non-phosphorylated substrate at a first rate and a phosphorylated substrate and a second rate. The output of the reporter compound is detected. In a preferred embodiment, the transferase activity detected is a kinase activity. In another preferred embodiment, the transferase activity detected is a phosphatase activity. Also provided is a method of screening for alterations in a transferase reaction. Kits and peptide substrate are also provided for carrying out at least one of the methods of the invention.
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申请公布号 |
US2004014159(A1) |
申请公布日期 |
2004.01.22 |
申请号 |
US20020199970 |
申请日期 |
2002.07.19 |
申请人 |
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发明人 |
GOUELI SAID A.;BULLEIT ROBERT F. |
分类号 |
C07K7/06;C07K7/08;C12Q1/37;C12Q1/48;G01N33/542;(IPC1-7):C12Q1/48 |
主分类号 |
C07K7/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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