发明名称 |
Promoters from chlorella virus genes providing for expression of genes in prokaryotic and eukaryotic hosts |
摘要 |
The invention is directed to novel promoters or mutants thereof from Chlorella virus DNA methyltansferase genes. A Chlorella virus gene promoter is operably linked to a first and/or second DNA sequence encoding a gene that is different from the Chlorella virus to form an expression cassette. An expression cassette can be introduced into prokaryotic and/or eukaryotic cells and can provide for a high level of expression of the gene encoded by the first and/or second DNA sequence. The invention also provides a method for screening other Chlorella virus genes for promoters that can function to express a heterologous gene in prokaryotic and/or eukaryotic hosts.
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申请公布号 |
US6673537(B1) |
申请公布日期 |
2004.01.06 |
申请号 |
US19990416548 |
申请日期 |
1999.10.12 |
申请人 |
BOARD OF REGENTS, UNIVERSITY OF NEBRASKA-LINCOLN |
发明人 |
MITRA AMITAVA;VAN ETTEN JAMES L. |
分类号 |
C12N9/10;C12N15/70;C12N15/74;C12N15/82;(IPC1-7):C12Q1/68;C12N15/00;C07H21/02;C07H21/04;A01H5/00 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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