NUCLEIC ACIDS AND METHODS FOR DETECTING VIRAL INFECTION, UNCOVERING ANTI-VIRAL DRUG CANDIDATES AND DETERMINING DRUG RESISTANCE OF VIRAL ISOLATES

摘要

A nucleic acid construct is provided. The nucleic acid construct includes an expression cassette including: a first polynucleotide region including a 5' NCR sequence of an RNA virus and at least an N-terminal portion of a core sequence of the RNA virus; a second polynucleotide region including a 3' UTR sequence of the RNA virus and at least a C-terminal portion of a polymerase sequence of the virus; and a third polynucleotide region encoding a reporter molecule, the third polynucleotide region being flanked by the first and the second polynucleotide regions; and a promoter sequence being operatively linked to the expression cassette in a manner so as to enable a transcription of a minus strand RNA molecule from the expression cassette.