| 摘要 |
The disclosed invention relates to a novel rPLA2 enzyme, it's antibody and use and the preparation method. The purified enzyme, derived from RBC cytosol, produces arachidonic acid dependently to calcium ion, has 42 kDa of M.W. determined by SDS-PAGE analysis, shows its isoeletric point at the pH ranging 3.9 to about 4.1 in electrophoresis analysis, has maximum activity at the pH ranging 9.5 to 10 and has its specific activity of 5.6 nM/min/mg. The antibody of disclosed invention reacts with the enzyme rPLA2 only and rPLA2 is inactivated by EA4 compound, rPLA2 inhibitor. The disclosed invention shows that the 42 kDa rPLA2 identified as a novel form of Ca<2+>-dependent PLA2 plays an important role in hemostasis, thrombosis and/or erythropoiesis through the Ca<2+>-dependent release of AA.
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