EXPRESSION VECTOR, HOST, FUSED PROTEIN, PROCESS FOR PRODUCING FUSED PROTEIN AND PROCESS FOR PRODUCING PROTEIN

摘要

It is intended to provide an expression vector, a host, a fused protein, a protein, a process for producing a fused protein and a process for producing a protein which prevent the formation of an inert and abnormal protein in producing a recombinant protein and thus make it possible to efficiently produce a target protein in the natural type (i.e., a soluble type) in a lar ge amount. Namely, an expression vector containing (a) a first coding region encoding a polypeptide having a molecular chaperon activity and at least one restriction enzyme site into which (b) a second coding region encoding a protein can be inserted. In the above expression vector, the first coding region is effectively linked to a promoter and the restriction enzyme site i s located within the same reading frame as the first coding region and in the downstream of the first coding region. Alternatively, the restriction enzyme site is located so that the second coding region having been inserted is effectively linked to a promoter and the first coding region is located with in the same reading frame as the second coding region and in the downstream of the second coding region.