| 摘要 |
PURPOSE: Provided is a method for making a column packing material for chromatography used in GPC and HPLC, which has a controllable particle size and porosity and excellent isomer separation efficiency. CONSTITUTION: The method for making a multi-functional column packing material used in both of GPC and HPLC comprises the steps of: mixing a monovinyl monomer with a divinyl monomer in a predetermined ratio; adding a small amount of template molecules; forming polymer particles having an uniform particle size by UV radiation or redox mechanism; and extracting and removing the template molecules to obtain a porous molecular marking polymer particles. In particular, the template molecules are an amino acid, a protein, a nucleic acid, a polysaccharide, an antibody, a vitamin, a steroid, an enzyme, a metal ion, or a drug.
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