Thermostable enzyme promoting the fidelity of thermostable DNA polymerases- for improvement of nucleic acid synthesis and amplification in vitro

摘要

Thermostable enzyme exhibiting 3'-exonuclease-activity but essentially no DNA polymerase activity where the enzyme enhances fidelity of an amplification process when added to a second enzyme exhibiting polymerase activity, where the thermostable enzyme is obtained from Archeoglobus fulgidus. Also described is a method of preparing or amplifying DNA using the above-described composition. Also described is a method for amplifying DNA using a thermostable enzyme exhibiting 3'-exonuclease-activity but essentially no DNA polymerase activity and obtained from Archeoglobus fulgidus which enzyme is not or only to a negligible extent active on linear single stranded DNA.