| 摘要 |
<p>This invention provides novel methods for the detection of single nucleotide polymorphisms (SNPs). In one embodiment, the method involves i) providing a nucleic acid primer complementary to a nucleic acid sequence adjacent to the location of the single nucleotide comprising said single nucleotide polymorphism wherein said primer is of sufficient length to initiate transcription by a nucleic acid polymerase; ii) performing a primer extension reaction using a nucleic acid polymerase, a chain terminator to produce one or more extension reaction products, wherein one or more components of said primer extension reaction are labeled with a redox-active label whereby one or more extension reaction products produced by the primer extension reaction are labeled with said redoxactive label; iii) detecting the extension reaction products by detecting the redox-active label, wherein detection of an extension reaction product longer than the nucleic acid primer indicates the presence of said single nucleotide polymorphism in said target nucleic acid.</p> |
