发明名称 |
OPTICAL BIOSENSOR WITH ENHANCED ACTIVITY RETENTION FOR DETECTION OF HALOGENATED ORGANIC COMPOUNDS |
摘要 |
A distal tip, and associated method of producing, of a biosensor ion sensing transducer for use in detecting an analyte comprising an halogenated organic compound in an environment such as soil or an aqueous environment. Features include: a biocomponent comprising at least one enzyme for carrying out a dehalogenation of the compound; the biocomponent is immobilized to a surface of the tip; a treatment of the biocomponent for maintaining a period of enzymatic efficacy; and the biocomponent stabilized by means preferably selected from the group consisting of crosslinking a surface of the immobilized biocomponent, crosslinking a polymer layer to the biocomponent, adding a gel-hardening agent to the biocomponent, adding a stabilizing agent to the biocomponent, and modifying a component of the immobilizing means. Immobilization can be carried out by: (a) entrapment within a hydrogel; (b) entrapment within a polymeric network; (c) (micro)encapsulation; (d) covalent-bonding; and (e) adsorption. The dehalogenase of the biocomponent may be selected from the group consisting of hydrolases, subclass EC 3.8, and lyases, subclass EC 4.5 as listed in Table 2A. |
申请公布号 |
WO03025627(A3) |
申请公布日期 |
2003.12.18 |
申请号 |
WO2002US17407 |
申请日期 |
2002.06.01 |
申请人 |
COLORADO STATE UNIVERSITY RESEARCH FOUNDATION;REARDON, KENNETH, F.;DAS, NEEMA |
发明人 |
REARDON, KENNETH, F.;DAS, NEEMA |
分类号 |
C12Q1/00;G01N21/77;G01N21/80 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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