| 摘要 |
A new technique to determine the extent of DNA methylation entails generating DNA fragments of a test sample by cleaving at methylation sites that are not methylated while sparing methylation sites in the DNA that are methylated. This approach provides enhanced sensitivity to differences of even a single methylated cytosine, within or outside of a CpG island, and yet it can be employed to ascertain the methylation status of a region of DNA comprising a CpG island, a DNA region comprising one or more CpG-containing islands, or even a large variety of DNA regions. |
