| 摘要 |
An electrophoretic focusing apparatus and method is provided for obtaining a preparative-scale, free-fluid electrophoretic separator with high resolution and an analytical capability commensurate with capillary zone electrophoresis. The present invention combines features of electrophoresis and isoelectric focusing to accomplish large scale purifications and fractionations not previously possible, and features a separation chamber bounded by precision-pore insulated screens, a plurality of purge chambers, a plurality of electrode chambers, and a plurality of pump means. The device of the invention is capable of high speed of separation through the use of high voltage gradients produced by low voltages applied across the narrow chamber. The present invention is also highly flexible, with operation in either a constant electric field, continuous flow mode or in a linearly varying electric field, batch mode, and both modes permit scanning of the sample fraction content and display in a conventional histogram format.
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