Production of pancreatic procarboxy-peptidase B, isoforms and muteins thereof, and their use

摘要

The preparation of carboxypeptidase B and procarboxypeptidase B. Also the use of procarboxypeptidase B for the preparation of carboxypeptidase B. Pancreatic carboxypeptidase B or an isoform or a mutein of carboxypeptidase B may be prepared by (a) expressing a natural or unnatural enzymatically inactive precursor form of carboxypeptidase B in a microorganism by secretion, (b) purifying the precursor form expressed by secretion, and (c) converting the purified precursor form into the active form by an enzymatic treatment. A nucleic acid construct and a host cell containing the construct are useful for preparing pancreatic carboxypeptidase B or an isoform or a mutein thereof by this method