Methods of suppressing gene expression

摘要

DNA constructs useful for suppressing the expression of targeted genes are provided. The constructs encode an RNA molecule with a stem-loop structure that is unrelated to the target gene and that is positioned distally to a sequence specific for the gene of interest. Methods of suppressing the expression of selected genes and transformed hosts also are described. In one embodiment, the DNA constructs and methods are used to selectively suppress gene expression in plants and plant cells.