NUCLEOTIDE SEQUENCE OF THREONINE OPERON DEREGULATED FROM ISOLEUCINE AND METHOD FOR PRODUCING L-THREONINE USING TRANSFORMED CELL CONTAINING THE SAME

摘要

PURPOSE: A nucleotide sequence of threonine operon deregulated from isoleucine and a method for producing L-threonine using a transformed cell containing the same nucleotide sequence are provided. The transformed cell can produce high concentration of L-threonine regardless of the presence of isoleuicine. CONSTITUTION: A nucleotide sequence of threonine operon of Escherichia coli in which nucleotides at position -56 to -18 or a portion thereof are deleted is provided, wherein the nucleotide sequence of 39 bp-removed threonine operon of Escherichia coli is represented by SEQ ID NO: 2. A recombinant vector containing the nucleotide sequence of SEQ ID NO: 2 is provided, wherein a vector pECCG 122 obtained from KFCC 10696 is used; and the vector is pTHR(+). A transformed cell which is produced by transforming a host cell with the recombinant vector pTHR(+) is provided, wherein the host cell is Escherichia sp. or Corynebacterium; and the host cell is Escherichia coli KCCM 10236. A method for producing L-threonine comprises culturing the transformed cell and recovering L-threonine from the cultured medium.