An automated method for measuring the development of a biofilm on a plurality of surfaces using a confocal imaging system including: a) a radiation source system for forming a beam of electromagnetic radiation comprising one or more wavelengths; b) an optical system for directing and focusing said beam onto one or more planes of the object; c) a detection system for detecting electromagnetic radiation emitted from the object and producing image data; and d) a scanning system for scanning the object in a plurality of planes with the electromagnetic radiation, the method comprising the steps of: i) growing said biofilm on said plurality of surfaces; ii) detecting the presence of one or more fluorescent moieties within the biofilm by scanning the biofilm with electromagnetic radiation in a plurality of planes to produce a plurality of images; and iii) analysing said images by means of a data processing system under the control of computer software to determine the structure of the biofilm.
申请公布号
WO03095995(A1)
申请公布日期
2003.11.20
申请号
WO2003GB01816
申请日期
2003.04.28
申请人
AMERSHAM BIOSCIENCES UK LIMITED;GOODYER, IAN, DAVID;LABARBE, RUDI, WILLY, ROGER;RUEHLMANN, DIETRICH, OTTO;STUBBS, SIMON, LAURENCE, JOHN