IN SITU GENERATION OF VIRAL VECTORS

摘要

Materials and methods useful in the in situ generation of viral vectors are described. Briefly, virus is liberated from cells by treatment with polysorbate and tri-butyl phosphate yielding non-viscous lysates with relatively little cell debris. Lysis is followed by step density ultracentrifugation to isolate crude virus. Finally, purification and helper removal is accomplished by dynamic banding ultracentrifugation.