发明名称 |
NOVEL CELL LINE PRODUCING A LARGE QUANTITY OF PLASMA TYPE PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE(PAF-AH) IN A STABLE MANNER, PREPARATION METHOD THEREOF AND PRODUCTION METHOD OF THE ENZYME, PAF-AH USING THE SAME |
摘要 |
PURPOSE: Provided are a novel cell line producing a large quantity of plasma type platelet-activating factor acetylhydrolase(PAF-AH) in a stable manner, a preparation method thereof and a production method of the enzyme, PAF-AH using the same. The enzyme, PAF-AH is useful for prevention and treatment of diseases mediated by PAF. CONSTITUTION: A novel cell line producing plasma type platelet-activating factor acetylhydrolase(PAF-AH) is produced by cloning bovine plasma type PAF-AH gene to an eukaryote overexpression vector pCR3.1 and transforming an animal cell with the cloned vector, wherein the bovine plasma type PAF-AH gene consists of nucleotides positioned at 757 to 2094 in the nucleotide sequence of SEQ ID NO: 3; the animal cell is HEK293; and the cell line is BGP8 (KCLRF-BP-00055). A mass production method of the PAF-AH comprises culturing the PAF-AH producing cell line BGP8 (KCLRF-BP-00055) in a medium, wherein the medium is a serum free medium or a protein free medium.
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申请公布号 |
KR20030086680(A) |
申请公布日期 |
2003.11.12 |
申请号 |
KR20020024801 |
申请日期 |
2002.05.06 |
申请人 |
BIOGENIA CO., LTD.;CHANG, HYEUN WOOK;LEE, TAE YOON |
发明人 |
CHANG, HYEUN WOOK;LEE, TAE YOON |
分类号 |
C12N5/16;(IPC1-7):C12N5/16 |
主分类号 |
C12N5/16 |
代理机构 |
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主权项 |
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地址 |
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