| 摘要 |
PURPOSE: Optimal eukaryotic expression vectors are provided, which have optimal conditions for gene therapy and DNA vaccine. Therefore, the vectors containing desired genes are used for gene therapy and DNA vaccine. CONSTITUTION: A foreign gene expression vector comprising ITR, pCMV, TPL, SV40 poly(A), SV40 enhancer, ITR and kan sequentially is provided, wherein the expression vector is pGX30 having the nucleotide sequence of SEQ ID NO: 9. A recombinant vector contains human interleukin-12 mutant(hIL-12m) gene operably linked to downstream of TPL in the vector pGX30, wherein the recombinant vector is pGX30-hIL-12m. Transformed E. coli DH5alpha/pGX30-hp35/IRES/hp40-N222L (KCTC 10232BP) is produced by transforming E. coli with the vector pGX30-hIL-12m. A foreign gene expression vector comprising ITR, pCMV, TPL, bGH poly(A), ITR and Amp sequentially is provided, wherein the expression vector is pACP20 having the nucleotide sequence of SEQ ID NO: 11. A recombinant vector contains hIL-12m gene operably linked to downstream of TPL in the vector pACP20, wherein the recombinant vector is pACP20-hIL-12m. Transformed E. coli DH5alpha/pACP20-hp35/IRES/hp40-N222L (KCTC 10233BP) is produced by transforming E. coli with the vector pACP20-hIL-12m.
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