| 摘要 |
The present invention provides a method for determining whether a T cell reacts with a predetermined Major Histocompatibility Complex (MHC) type. In the method, a sample comprising said T cell is brought into contat with a plurality of molecules of said MHC type, each MHC molecule being complexed with a peptide antigen whose contribution to a T cell receptor binding the MHC-peptide antigen complex is minimised. Whether said plurality of MHC molecules causes activation of the T cell is then determined. The method can assess allo-specific T cell activity, and may be used in matching transplant and donor patients, as well as in monitoring alloreactive responses following a transplant operation. |
