HIGHLY SENSITIVE METHODS FOR PROTEIN DETECTION IN PROTEOMICS

摘要

The present invention relates to methods for identification and quantification of proteins expressed within a cell. The methods of the invention involve the separation of proteins based on their physical properties such as, for example, net charge, molecular weight, or immunoreactivity, followed by detection of said proteins using a number of different techniques including (i) ramification-extension amplification method (RAM); (ii) hybridization signal amplification method (HSAM); and (iii) detection with nanodots. The methods of the invention will have a variety of different uses including, but mot limited to, uses for screening, for diagnosis and prognosis of disease. The method of the invention are especially useful for identification of proteins that are identified due to the size of the protein, low concentration of the protein, or failure to separate proteins due to similar physical properties.