METHODS FOR GENE TRANSFER USING PSEUDOTYPED LENTIVIRUSES

摘要

Methods for introducing nucleic acid sequences into hepatocytes, brain glial cells and airway epithelial cell are provided. The methods use filoviral and togaviral glycoprotein-pseudotyped lentiviruses. The viruses comprise a lentiviral capsid and a viral envelope further comprising a lipid bilayer and a functional filoviral glycoprotein or two functional togaviral glycoproteins. In one embodiment the lentivirus is a feline immunodeficiency virus (FIV). In an alternate embodiment, the filoviral glycoprotein is a Marburg virus glycoprotein. In another embodiment, the Marburg virus glycoprotein can have a mutation in the C-terminal portion of the amino acid sequence that results in a higher titer production of the pseudotyped virus. In an alternate embodiment the togaviral glycoproteins are alphavirus glycoproteins, for example, the E1 and the E2 envelope glycoproteins of Ross River virus (RRV).