发明名称 |
Methods for identifying compounds which inhibit binding of nucleocapsid 7 protein to HIV-1 RNA |
摘要 |
The present invention relates to methods of identifying a molecule from a library of molecules that inhibits binding of human immunodeficiency virus nucleocapsid 7 polypeptide (NCp7) to an oligonucleotide which comprises admixing an NCp7 polypeptide with at one labeled HIV-1 psi-site oligonucleotide and an amount of the molecule to be tested under binding conditions; and determining the amount of oligonucleotide bound to the NCp7 polypeptide, wherein a decrease in the amount of oligonucleotide bound in the presence of the molecule compared with the amount of oligonucleotide bound in the absence of the molecule indicates that the molecule inhibits binding of NCp7 polypeptide to the oligonucleotide.
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申请公布号 |
US2003198648(A1) |
申请公布日期 |
2003.10.23 |
申请号 |
US20030339217 |
申请日期 |
2003.01.09 |
申请人 |
BUECHTER DOUGLAS;HOU XIAOHONG;RICE WILLIAM G.;MARLOR CHRISTOPHER W.;YANG WENGANG |
发明人 |
BUECHTER DOUGLAS;HOU XIAOHONG;RICE WILLIAM G.;MARLOR CHRISTOPHER W.;YANG WENGANG |
分类号 |
C07K14/16;G01N33/569;(IPC1-7):A61K39/395;A61K39/12;A61K39/21 |
主分类号 |
C07K14/16 |
代理机构 |
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地址 |
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