IN VITRO ASSEMBLY OF SV40 VIRUSES AND PSEUDOVIRUSES

摘要

The present invention relates to a method for the improved in vitro assembly of pseudoviruses. These viruses or pseudoviruses comprise at least one pure or semi-purified SV40 capsid protein and at least one exogenous constituent selected from nucleic acids, proteins and peptides. In the method of the invention at least one semi-purified or pure SV40 capsid protein is brought into contact with at least one exogenous constituent in the presence of semi-purified or pure PARP [Poly (ADP-ribose) polymerase]. The invention further relates to use of pseudoviruses assembled by the method of the invention, in the preparation of a pharmaceutical composition for the treatment of an individual suffering from pathological disorder.