发明名称 |
PROCESS FOR PRODUCING RECOMBINANT PROTEIN AND FUSED PROTEIN |
摘要 |
This invention provides a recombinant protein expression system using a host and a cell-free translation system capable of universally expressing a large amount of any proteins as soluble proteins while preventing expression of the toxicity of a desired protein in hosts, formation of inclusion bodies and decomposition with proteases by expressing the desired protein as a fusion protein with chaperonins, that are, about 60 kDa molecular chaperones, 60 kDa heat shock proteins, or thermosomes and accommodating it certainly in the inside of a stereostructure of a chaperonin. <??>A process for producing a protein, which comprises transcribing and translating a gene containing a gene encoding the linked chaperonin subunits and a gene encoding a desired protein thereby synthesizing a fusion protein having the desired protein linked via a peptide linkage to the linked chaperonin subunits.
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申请公布号 |
EP1354959(A1) |
申请公布日期 |
2003.10.22 |
申请号 |
EP20010272326 |
申请日期 |
2001.12.26 |
申请人 |
SEKISUI CHEMICAL CO., LTD.;MARINE BIOTECHNOLOGY INSTITUTE CO., LTD. |
发明人 |
FURUTANI, MASAHIRO;HATA, JUNICHI;TOGI, AKIKO |
分类号 |
C07K19/00;C12N15/62;C12N15/70;C12P21/02;(IPC1-7):C12P21/02;C12N15/09 |
主分类号 |
C07K19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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