METHOD OF DESIGNING SYNTHETIC NUCLEIC ACID SEQUENCES FOR OPTIMAL PROTEIN EXPRESSION IN A HOST CELL

摘要

The present invention provides a method for modifying a wild type nucleic ac id sequence encoding a polypeptide to enhance expression and accumulation of th e polypeptide in the host cell by harmonizing synonymous codon usage frequency between the foreign DNA and the host cell DNA. This can be done by substituting codons in the foreign coding sequence with codons of similar usage frequency from the host DNA/RNA which code for the same amino acid. Th e present invention also provides novel synthetic nucleic acid sequences prepared by the method of the invention.