| 摘要 |
The invention relates to a method for detecting cytosine-methylation in DNA samples which consists of the following steps: firstly, a genomic DNA sample, which comprises background DNA and DNA which is to be examined, is chemically treated in such a way that all non-methylated cytosine bases are converted into uracil, whereby the 5-methyl cytosine bases remain unchanged. The DNA sample which has been chemically treated is then amplified by using at least 2 primer-oligo-nucleotides and a polymerase, the DNA which is to be examined with respect to the background DNA is preferred as a template. In the final step, the amplificates are analysed and on the basis of the presence of an amplificate and/or from the analysis of further positions information on the methylation status is deduced in the DNA which is to be examined. |
