发明名称 |
POST-SYNTHESIS LABELING OF NUCLEIC ACIDS AND USES THEREOF |
摘要 |
An assay is provided for nucleic acids that can be post-synthetically labeled, wherein modified nucleoside triphosphates are used that are more efficiently and specifically incorporated during nucleic acid synthesis than labeled nucleoside triphosphates. In a preferred embodiment, nucleoside a-thiotriphosphates are utilized. Maleimide or iodoacetamide conjugating moieties can be attached post-synthetically. The conjugating moieties may include a reporter group. Also disclosed are new methods for detecting single nucleotide polymorphism. |
申请公布号 |
WO03052115(A3) |
申请公布日期 |
2003.10.09 |
申请号 |
WO2002SE02331 |
申请日期 |
2002.12.13 |
申请人 |
AMERSHAM BIOSCIENCES AB |
发明人 |
BRUSH, CHARLES, K.;GUPTA, VINEET |
分类号 |
G01N33/53;C12M1/00;C12N15/09;C12Q1/68;G01N33/566 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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